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1.
J Pers Soc Psychol ; 2024 Feb 29.
Artigo em Inglês | MEDLINE | ID: mdl-38421749

RESUMO

Self-control-the prioritization of valued global goals over immediate local rewards-is typically conceptualized and studied as isolated decisions. Goal pursuit, however, generally requires people to make repeated self-control decisions across contexts. We adopt a higher order, strategic level of analysis of self-control and explore, for the first time, people's preferences for abstinence (a pattern of choices in which one never indulges) versus moderation (a pattern of choices in which one indulges when doing so does not harm one's goals or even helps promote the pursuit of those goals). To understand when and why people may opt for one over the other, the present work explores one psychological feature that may support these strategy preferences: the representation of self-control conflicts as inherent (i.e., choice options are mutually contradictory) versus situational (i.e., choice options compete for limited resources). We present eight studies in the main text and three in the online Supplemental Materials documenting that people associate inherent and situational conflict representations with abstinence and moderation, respectively. By documenting that strategy preferences may differ as a function of conflict representations, this work questions the assumption of abstinence as the primary indicator of self-control success, raises methodological and conceptual questions about how best to assess these strategy preferences, and calls for greater understanding of self-control as a recurrent decision-making process. (PsycInfo Database Record (c) 2024 APA, all rights reserved).

2.
Transl Behav Med ; 2023 Nov 27.
Artigo em Inglês | MEDLINE | ID: mdl-38011653

RESUMO

Early slow weight loss during treatment is associated with less weight loss overall. The impact of an augmented intervention designed for early slow weight loss responders compared with a standard diabetes prevention intervention was evaluated following 12 months of treatment and 6 months of no contact. The impact of standard vs. augmented intervention sequences on weight and glycemia also was determined. Adults were ≥21 years old with overweight or obesity and prediabetes (n = 174). Slow responders were stratified to augmented treatment if they failed to achieve >2.5% weight loss (%WL) at Week 5. Matched within-sex pairs of participants were created based on %WL at Month 5 following the intensive intervention phase, and each person within the pair was randomly assigned to treatment for Months 5-12 during the extended intervention phase. Both 12-month interventions included a ≥7%WL goal. Mean 12-month %WL was 5.29% (95% CI: 4.27%-6.31%; P < .0001) and 18-month %WL was 3.34% (95% CI: 2.01%-4.66%; P < .0001) overall. %WL was greater for the standard (9.55%) than the augmented (4.0%) intervention (P = .0001); no differences occurred in weight regain between early and slow responders (P = .9476). No differences occurred in mean %WL at 12 months between the standard and augmented groups after controlling for %WL at Week 5 and sex (P = .23) nor in the change in glycemia (all P > .05). WL following the first month of treatment predicted 12- and 18-month WL success regardless of intervention sequence; however, even early slow responders achieved significant WL during treatment. Further research is needed to support effective WL maintenance for people with prediabetes.


Weight loss is a primary strategy for risk reduction in adults with prediabetes, and early weight loss may indicate weight loss success long-term. Early slow weight loss responders during behavioral treatment may benefit from alternate treatment compared with remaining in a standard diabetes prevention program. An intervention augmented with training in goal setting and problem-solving was implemented among slow weight loss responders following the first month of treatment in the current study. The change in percent weight loss observed in the augmented intervention compared with the standard diabetes prevention intervention was determined at 12 and 18 months from baseline. Both the standard and augmented interventions facilitated significant weight loss at 12 months. Participants who lost more than 2.5% of their weight during the first month of treatment (early responders) lost more weight overall during the study compared with people who were slower to respond. Percent weight loss following the first month of lifestyle intervention and sex predicted percent weight loss at 12 months. Participants regained some weight at 18 months regardless of the treatment group but weighed less than their baseline weight. Both early and slow weight loss responders may benefit from ongoing support following 12 months of treatment to achieve weight loss maintenance.

3.
Front Psychol ; 14: 1271795, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-37663342

RESUMO

[This corrects the article DOI: 10.3389/fpsyg.2023.1124171.].

4.
J Pestic Sci ; 48(3): 71-77, 2023 Aug 20.
Artigo em Inglês | MEDLINE | ID: mdl-37745171

RESUMO

The Cucurbitaceae family accumulates hydrophobic organic pollutants in its aerial parts at high concentrations. Major latex-like proteins (MLPs) were identified in zucchini (Cucurbita pepo) as a transporting factor for hydrophobic organic pollutants. MLPs bind to hydrophobic organic pollutants in the roots, are secreted to xylem vessels as complexes, and are transported to the aerial parts. However, the suitable conditions for binding MLPs to hydrophobic organic pollutants remain elusive. In the present study, we show that MLPs bind to the hydrophobic organic pollutant pyrene with higher affinity under acidic conditions. Our results demonstrated that pH regulates the binding of MLPs to hydrophobic organic pollutants.

5.
J Exp Psychol Gen ; 152(12): 3526-3545, 2023 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-37676129

RESUMO

Growth mindsets are beliefs that abilities, like intelligence, are mutable. Although most prior work has focused on people's personal mindset beliefs, a burgeoning literature has identified that organizations also vary in the extent to which they communicate and endorse growth mindsets. Organizational growth mindsets have powerful effects on belonging and interest in joining organizations, suggesting that they may be a productive way to intervene to improve individual and societal outcomes. Yet, little is known about for whom organizational mindset interventions might be more or less effective, a critical question for effective implementation and theory. We examine whether people's personal mindset beliefs might determine the effect of organizational growth mindsets, and if so, whether this moderation reflects a matching or mismatching pattern. Three experiments manipulated the espoused mindset of an organization and found that organizational growth mindsets primarily increased belonging and interest in joining among participants who personally endorsed matching growth mindset beliefs. An additional field study provided ecological validity to these findings, replicating them with students' experiences of belonging in classrooms. This study also revealed a divergent mismatching pattern on grades: rather than bolstering the grades of students with growth mindsets, growth mindset classroom contexts primarily enhanced the grades of students with more fixed mindsets. By clarifying for whom organizational growth mindsets are beneficial and in what manner, the current work provides theoretical and practical insight into the psychological dynamics of organizational growth mindsets. (PsycInfo Database Record (c) 2023 APA, all rights reserved).


Assuntos
Inteligência , Estudantes , Humanos , Estudantes/psicologia
6.
J Plant Physiol ; 287: 154038, 2023 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-37413840

RESUMO

Xylem sap is a fluid that transfers water and nutrients from the rhizosphere. This sap contains relatively low concentrations of proteins that originate from the extracellular space among the root cells. One of the characteristic proteins in the xylem sap of the Cucurbitaceae family, which includes cucumber and zucchini, is a major latex-like protein (MLP). MLPs are responsible for crop contamination through the transport of hydrophobic pollutants from the roots. However, detailed information on the content of MLPs in the xylem sap is not available. Proteomic analysis of root and xylem sap proteins from the Cucurbita pepo cultivars Patty Green (PG) and Raven (RA) showed that the xylem sap of cv. RA, a high accumulator of hydrophobic pollutants, contained four MLPs that accounted for over 85% of the total xylem sap proteins in this cultivar. The xylem sap of PG, a low accumulator, mainly contained an uncharacterized protein. The amount of each root protein between the PG and RA cultivars was significantly and positively correlated in spite of being with and without a signal peptide (SP). However, the amount of xylem sap proteins without an SP was not correlated. These results suggest that cv. RA is characterized by MLPs in the xylem sap.

7.
Front Psychol ; 14: 1124171, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-37359877

RESUMO

Self-regulation research highlights the performance trade-offs of different motivational states. For instance, within the context of regulatory focus theory, promotion motivation enhances performance on eager tasks and prevention motivation enhances performance on vigilant tasks (i.e., regulatory focus task-motivation fit). Work on metamotivation-people's understanding and regulation of their motivational states-reveals that, on average, people demonstrate knowledge of how to create such task-motivation fit; at the same time, there is substantial variability in this normative accuracy. The present research examines whether having accurate normative metamotivational knowledge predicts performance. Results revealed that more accurate metamotivational knowledge predicts better performance on brief, single-shot tasks (Study 1) and in a consequential setting (course grades; Study 2). The effect was more robust in Study 2; potential implications of this variability are discussed for understanding when and why knowledge may be associated with performance.

8.
Am J Health Behav ; 47(2): 337-348, 2023 04 30.
Artigo em Inglês | MEDLINE | ID: mdl-37226352

RESUMO

Objectives: Males often lose more weight than females during treatment, and early weight loss predicts weight loss longer-term. Yet, mechanisms for sex differences in early weight loss are unknown and were examined in this study.Methods: Adults≥21 years old with overweight or obesity and prediabetes (N=206) participated in a lifestyle intervention and completed baseline psychosocial questionnaires. Percent weight loss, session attendance, and number of days participants self-monitored dietary intake and weight were determined at week 5. Principal components, regression, and mediation analyses were conducted to determine whether weight loss differed by sex and potential mediators of weight change. Results: Mean (±SD) weight loss was greater for males (2.59±1.62%) than females (2.05±1.54%; p=.02). Attendance, self-monitoring, and beliefs regarding disease risk were independent predictors of weight loss (all p<.05) but did not explain sex differences. The association between attendance and weight loss was stronger for males than females (p<.05). Conclusions: Additional research is needed to identify mechanisms that explain sex differences in early weight loss. However, strengthening risk beliefs, attendance, and self-monitoring may promote greater early weight loss for all participants.


Assuntos
Estado Pré-Diabético , Caracteres Sexuais , Adulto , Humanos , Feminino , Masculino , Adulto Jovem , Estilo de Vida , Obesidade/prevenção & controle , Estado Pré-Diabético/terapia , Redução de Peso
9.
J Behav Med ; 46(5): 770-780, 2023 10.
Artigo em Inglês | MEDLINE | ID: mdl-36933057

RESUMO

Self-regulation can facilitate modifications in lifestyle to promote behavioral change. However, little is known about whether adaptive interventions promote improvement in self-regulatory, dietary, and physical activity outcomes among slow treatment responders. A stratified design with an adaptive intervention for slow responders was implemented and evaluated. Adults ≥ 21 years old with prediabetes were stratified to the standard Group Lifestyle Balance intervention (GLB; n = 79) or the adaptive GLB Plus intervention (GLB + ; n = 105) based on first-month treatment response. Intake of total fat was the only study measure that significantly differed between groups at baseline (P = 0.0071). GLB reported greater improvement in self-efficacy for lifestyle behaviors, goal satisfaction with weight loss, and very active minutes of activity than GLB + (all P < 0.01) at 4-months. Both groups reported significant improvement in self-regulatory outcomes and reduction in energy and fat intake (all P < 0.01). An adaptive intervention can improve self-regulation and dietary intake when tailored to early slow treatment responders.


Assuntos
Diabetes Mellitus , Estado Pré-Diabético , Adulto , Humanos , Adulto Jovem , Estado Pré-Diabético/terapia , Dieta , Diabetes Mellitus/prevenção & controle , Exercício Físico/fisiologia , Estilo de Vida
10.
J Pers Soc Psychol ; 124(4): 796-811, 2023 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-35862084

RESUMO

From whom do people seek what type of support? Although people regularly seek support from close and distant others, little work has systematically investigated when and why people approach different people in their support network for different types of support. The present research introduces a novel distinction of social support and explores its relationship to the scope or range of support providers people would consider asking for support. Based on a recent extension of construal level theory (Trope et al., 2021), five experiments tested the bidirectional relation between levels of support and scope-the latter assessed by the social distance of potential support providers. Experiment 1 demonstrated that people can categorize supportive behaviors into low-level support (i.e., addressing the effect of a problem) and high-level support (i.e., addressing the cause of a problem). Experiments 2 and 4 showed that being prompted to seek low-level (vs. high-level) support-oriented people toward support providers who are socially proximal (vs. distal). In Experiment 3, thinking about interacting with a socially proximal (vs. distal) support provider led to a greater focus on receiving low-level (vs. high-level) support. Testing the implication of the link between levels of support and scope, Experiment 5 demonstrated that support recipients reported they would feel more gratitude when they imagined receiving low-level (vs. high-level) support from socially proximal (vs. distal) support providers. Broader implications for social support, interpersonal relationships, and construal level theory research are discussed. (PsycInfo Database Record (c) 2023 APA, all rights reserved).


Assuntos
Emoções , Relações Interpessoais , Humanos , Apoio Social
11.
Emotion ; 23(1): 138-162, 2023 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-34780240

RESUMO

How do people think about happiness? Is it something best enjoyed as an investment over time, or is it something fleeting that should be savored? When people view happiness as an investment, they may endorse delaying happiness (DH)-the belief that working hard and sacrificing opportunities for happiness now will contribute to greater future happiness. When people view happiness as fleeting, they may endorse living in the moment (LM)-the belief that one should seize proximal opportunities to experience happiness now, rather than later. Using a mix of cross-sectional, meta-analytic (Studies 1, 2a, 2b, 2c), experimental (Study 3), and daily diary methods (Study 4), people who endorsed DH or LM beliefs anticipated more positive affect upon goal attainment and experienced greater well-being, but only DH was related to more negative affect when pursuing nonfocal goals and less delay discounting of future rewards. Implications for self-regulation and emotion are discussed. (PsycInfo Database Record (c) 2023 APA, all rights reserved).


Assuntos
Emoções , Felicidade , Humanos , Estudos Transversais , Emoções/fisiologia , Gerenciamento de Dados
12.
Pers Soc Psychol Bull ; 49(11): 1615-1632, 2023 11.
Artigo em Inglês | MEDLINE | ID: mdl-36065608

RESUMO

Self-affirmation-reflecting on a source of global self-integrity outside of the threatened domain-can mitigate self-threat in education, health, relationships, and more. Whether people recognize these benefits is unknown. Inspired by the metamotivational approach, we examined people's beliefs about the benefits of self-affirmation and whether individual differences in these beliefs predict how people cope with self-threat. The current research revealed that people recognize that self-affirmation is selectively helpful for self-threat situations compared with other negative situations. However, people on average did not distinguish between self-affirmation and alternative strategies for coping with self-threat. Importantly, individual differences in these beliefs predicted coping decisions: Those who recognized the benefits of self-affirmation were more likely to choose to self-affirm rather than engage in an alternative strategy following an experience of self-threat. We discuss implications for self-affirmation theory and developing interventions to promote adaptive responses to self-threat.


Assuntos
Adaptação Psicológica , Autoimagem , Humanos
13.
Pers Soc Psychol Bull ; : 1461672221119356, 2022 Oct 11.
Artigo em Inglês | MEDLINE | ID: mdl-36218360

RESUMO

People with financially contingent self-worth (FCSW) base their self-esteem on money and feel pressured to achieve financial success. However, the present research suggests such individuals may be vulnerable to compulsive buying and experiencing distress and impairment in their lives from engaging in this maladaptive behavior (Study 1a-1b). Study 2 identified a key mechanism: People with FCSW experience more motivational conflict between wanting to spend (vs. not spend) their money, which predicts greater compulsive buying intentions and anticipated distress from making excessive purchases. A 5-week diary study revealed that FCSW-on average and at a weekly level-predicted greater perceived financial motivational conflict and more compulsive buying, distress, and impairment in life (Study 3). People with FCSW experience more financial motivational conflict, independent of beliefs about spending implying wealth or feeling pressured to spend to display one's wealth to others (Study 4). Implications and future directions are discussed.

14.
Diabetes Care ; 45(10): 2452-2455, 2022 10 01.
Artigo em Inglês | MEDLINE | ID: mdl-36041053

RESUMO

OBJECTIVE: Some people are slower to respond during lifestyle interventions. An adaptive "rescue" intervention may improve outcomes among slow responders. The impact of a worksite rescue intervention for early slow responders was evaluated. RESEARCH DESIGN AND METHODS: Employees ≥21 years old with prediabetes were stratified to intervention using a 2.5% weight loss (%WL) threshold at week 5. Outcomes were assessed at baseline and at 4 months using mixed-effect and linear regression models. RESULTS: Significant improvement occurred in mean %WL, glycemia, total cholesterol, and triglycerides in the standard compared with the adaptive (Group Lifestyle Balance Plus [GLB+]) intervention (all P≤ 0.01). However, GLB+ participants also experienced a significant reduction in %WL and glycemia (all P < 0.01). The %WL at week 5 significantly predicted %WL at 4 months (P < 0.0001). The between-group difference of 4-month %WL was not significant for someone achieving 2.5%WL at week 5. CONCLUSIONS: Diabetes prevention programs should consider weight loss success following 1 month of treatment and offer a rescue intervention to early slow weight loss responders.


Assuntos
Diabetes Mellitus , Estado Pré-Diabético , Adulto , Colesterol , Humanos , Estado Pré-Diabético/terapia , Triglicerídeos , Redução de Peso , Adulto Jovem
15.
Arthrosc Tech ; 11(5): e841-e846, 2022 May.
Artigo em Inglês | MEDLINE | ID: mdl-35646559

RESUMO

Osgood-Schlatter disease commonly affects physically active adolescents. It is a common cause of anterior knee pain and inflammation in this population. Its symptoms typically subside with conservative therapy. Surgery, including resection of mobile ossicles, is considered when the pain persists on kneeling or during sports after the skeletal maturity. In this procedure, we use a direct bursoscopic approach with ultrasound-guided ossicle resection. In comparison with the classical arthroscopic approach, the bursoscopic approach uses more distally placed portals. These reduce the risk of damage to the fat pad, meniscus, and ligament. Endoscopic surgeries, including arthroscopic and bursoscopic surgeries, use intraoperative fluoroscopy to resect ossicles because the ossicle cannot be clearly identified by endoscopic imaging alone. Fluoroscopy exposes patients and surgeons to radiation. Ultrasound-guided surgery identifies the exact positional relationship between the ossicle and grasping forceps without radiation exposure since fluoroscopy is unnecessary. Moreover, the risk of residual ossicles is reduced because tiny ossicles, which are difficult to detect under fluoroscopy, are visible on ultrasound. Ultrasound-guided ossicle resection was a viable treatment option for Osgood-Schlatter disease because it eliminated radiation exposure and reduced the risk of missed ossicles.

16.
Mol Biol Rep ; 49(8): 7773-7782, 2022 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-35648252

RESUMO

BACKGROUND: Zucchini plants (Cucurbita pepo) accumulate persistent organic pollutants (POPs) at high concentrations in their aerial parts, and major latex-like proteins (MLPs) play crucial roles in their accumulation. MLPs bind to POPs in root cells, MLP-POP complexes are then translocated into xylem vessels, and POPs are transported to the aerial parts. We previously identified three CpMLP genes (MLP-PG1, MLP-GR1, and MLP-GR3) as transporting factors for POPs; however, other studies have shown that the genomes of several plant species contain more than 10 MLP genes, thus, further MLP genes responsible for POP accumulation may have been overlooked. METHODS AND RESULTS: Here, we investigated the number of CpMLP genes by performing a hidden Markov model search against the C. pepo genome database and characterized their effects on POP accumulation by performing the expression analysis in the organs and in silico structural analysis. The C. pepo genome contained 21 CpMLP genes, and several CpMLP genes, including MLP-PG1 and MLP-GR3, were highly expressed in roots. 3D structural prediction showed that all examined CpMLPs contained a cavity with a hydrophobic region, which facilitated binding to POPs. CONCLUSIONS: The present study provides insights regarding CpMLP genes responsible for POP accumulation.


Assuntos
Cucurbita , Poluentes do Solo , Biodegradação Ambiental , Cucurbita/genética , Látex/análise , Látex/metabolismo , Raízes de Plantas/metabolismo , Poluentes do Solo/análise
17.
Chemosphere ; 305: 135536, 2022 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-35772518

RESUMO

The Cucurbitaceae family accumulates dioxin-like compounds in its fruits. We previously showed that A20/AN1 zinc finger protein (ZFP) genes were highly expressed in the zucchini (Cucurbita pepo) subspecies pepo, which accumulates dioxin-like compounds at high concentrations. Transgenic tobacco (Nicotiana tabacum) plants overexpressing A20/AN1 ZFP genes show accumulation of dioxin-like compounds in their upper parts. However, the mechanisms underlying the accumulation of dioxin-like compounds regulated by the A20/AN1 ZFPs remain unclear. Here, we show that A20/AN1 ZFPs positively regulate the expression of the major latex-like protein (MLP) and its homolog genes in N. tabacum and C. pepo. MLPs are involved in the transport of dioxin-like compounds from the roots to the upper parts of C. pepo. Overexpression of A20/AN1 ZFP genes in N. tabacum leads to the upregulation of pathogenesis-related protein class-10 genes with the binding ability toward dioxin-like compounds. Our results demonstrated that A20/AN1 ZFPs upregulate MLP and its homolog genes in N. tabacum and C. pepo, resulting in the accumulation of dioxin-like compounds.


Assuntos
Cucurbita , Dioxinas , Cucurbita/genética , Cucurbita/metabolismo , Dioxinas/metabolismo , Látex , Nicotiana/genética , Zinco/metabolismo , Dedos de Zinco/genética
18.
Vaccine ; 40(33): 4922-4928, 2022 08 05.
Artigo em Inglês | MEDLINE | ID: mdl-35690499

RESUMO

BACKGROUND: Japan amended the recommended age for the Bacille Calmette-Guérin (BCG) vaccination to less than 6 months after 2005, but subsequently amended the recommended age to 5-8 months (latest amendment, <1 year) in April 2013 due to the increasing incidence of BCG-associated osteitis/osteomyelitis since 2005. METHODS: We collected data on BCG-associated vaccine adverse events (VAEs) in the population aged <1 year between April 2013 and March 2017. The incidence of BCG-associated VAE was analyzed using census and vaccine coverage data from the government website. We compared the incidence of VAEs in patients vaccinated at less than 6 months with those vaccinated at 6 months or older. RESULTS: Among the 581 BCG-associated VAEs recorded during the study period, 354 (61%) were male, and the average age at vaccination was 5.7 months. The incidence of VAEs per million population aged <1 year at vaccination was highest for suppurative lymphadenitis (63.7), followed by skin lesions (38.4), and BCG-associated osteitis/osteomyelitis (3.1). Disseminated BCG and anaphylaxis were rare (1.1 and 1.6%, respectively). The incidence of VAEs in the population vaccinated at <6 months of age was higher for BCG-associated osteitis/osteomyelitis (3.8) and disseminated BCG (1.3) than in the population vaccinated at ≥6 months. CONCLUSIONS: The population vaccinated at <6 months of age was more likely to develop BCG-associated osteitis/osteomyelitis than the population vaccinated at ≥6 months of age, indicating that the change in the recommended vaccination age in 2013 might have contributed to the subsequent decrease in the incidence of BCG-associated osteitis/osteomyelitis.


Assuntos
Vacina BCG , Osteíte , Osteomielite , Vacina BCG/efeitos adversos , Feminino , Humanos , Incidência , Lactente , Japão/epidemiologia , Masculino , Osteíte/etiologia , Osteomielite/induzido quimicamente , Osteomielite/complicações , Vacinação/efeitos adversos
19.
Pers Soc Psychol Bull ; 48(5): 807-820, 2022 05.
Artigo em Inglês | MEDLINE | ID: mdl-34238062

RESUMO

Recent metamotivation research revealed that Westerners recognize that promotion versus prevention motivations benefit performance on eager versus vigilant tasks, respectively; that is, they know how to create task-motivation fit with respect to regulatory focus. Westerners also believe that, across tasks, promotion is more beneficial than prevention (i.e., a promotion bias). Adopting a cross-cultural approach, we examined whether beliefs about task-motivation fit generalize across cultures, whether Easterners exhibit a contrasting prevention bias, and the role of independence/interdependence in these beliefs. Results revealed cross-cultural similarities in metamotivational beliefs. Moreover, Easterners and Westerners alike often exhibited a promotion bias, suggesting that this effect may not be shaped by culture. One potential cultural difference did emerge: Easterners appeared to recognize how to create task-motivation fit for both independent and interdependent outcomes, whereas Westerners only recognized how to do so for independent outcomes. We discuss the role of culture in shaping metamotivation.


Assuntos
Comparação Transcultural , Motivação , Viés , Humanos
20.
Planta ; 255(1): 10, 2021 Nov 30.
Artigo em Inglês | MEDLINE | ID: mdl-34850294

RESUMO

MAIN CONCLUSION: MLP-PG1, identified in Cucurbita pepo, plays a crucial role in resistance against fungal pathogens through the induction of pathogenesis-related genes. ASTRACT: MLP-PG1, a major latex-like protein (MLP) from zucchini (Cucurbita pepo), was identified as a transporting factor for hydrophobic organic pollutants. MLPs are members of the Bet v 1 family, similar to pathogenesis-related class 10 proteins (PR-10s). However, the biological functions of MLPs remain unclear. Herein, we show that MLP-PG1 induces the expression of pathogenesis-related (PR) genes and indirectly promotes resistance against pathogens. The activity of the MLP-PG1 promoter in leaves of transgenic tobacco plants was significantly enhanced by inoculation with Pseudomonas syringae pv. tabaci. However, MLP-PG1 did not induce direct resistance through RNase activity. Therefore, we examined the possibility that MLP-PG1 is indirectly involved in resistance; indeed, we found that MLP-PG1 induced the expression of defense-related genes. Overexpression of MLP-PG1 highly upregulated PR-2 and PR-5 and decreased the area of lesions caused by Botrytis cinerea in the leaves of transgenic tobacco plants. Our results demonstrate that MLP-PG1 is involved in indirect resistance against plant diseases, especially caused by fungal pathogens, through the induction of PR genes. This study is the first report to show the induction of PR genes by the expression of MLP from the RNA sequencing analysis and the involvement of MLP-PG1 in the resistance.


Assuntos
Cucurbita , Cucurbita/genética , Látex , Plantas Geneticamente Modificadas , Pseudomonas syringae , Nicotiana/genética
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